Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLTCFKAYDIRGKLGTELNEEIAYKIGRAYGQIYK-----PKTVVVGCDIRLSSEALKQAAIRGLNDAGVNVLDLGMTGTEEVYFAAFHLDVQGGIEVTASHNPMDYNGMKLVRENARPISADTGLKEIQALAETNNFEEV--GQKGTTQSYNILPEFVDHLLTYIEPAK--IRPLKLVVNAGNGAAGHVIDAIEEKFKALNVPVEFIKIHHEADGTFPNGIPNPILIENRDSTRNAVLEHKADMGIAWDGDFDRCFLFDEKGQFIEGYYIVGLLAQAFLIKQSGEKIVHDPRLVWNTFDIVDEYKGVTVQSKSGHAFIKDVMREHNAVYGGEMSAHHYFRDFAYCDSGMIPWLLTIVLLSETGQSLSTLVENMIAKFPCSGEINFKVADTQTTIQKIFDFYADQNPQIDRTDGVSLNFGAWRFNVRASNTEPLLRLNIESRADRQAQPMQYYVDELTGLIQN 1WQA Chain:D ((3-450)) ----KLFGTFGVRGIANEKITPEFAMKIGMAFGTLLKREGRKKPLVVVGRDTRVSGEMLKEALISGLLSVGCDVIDVGIAPTPAVQWATKHFNADGGAVITASHNPPEYNGIKLLEPNGMGLKKER-EAIVEELFFKEDFDRAKWYEIGEVRREDIIKPYIEAIKSKVDVEAIKKRKPFVVVDTSNGAGSLTLPYLLREL---G--CKVITVNAQPDGYFPARNPEPNE-ENLKEFMEIVKALGADFGVAQDGDADRAVFIDENGRFIQGDKTFALVADAVLKEKGGGLLVTTVATSNLLDDIAKKHGAKVMRTKVGDLIVARALYENNGTIGGEENGGVIFPEHVLGRDGAMTVAKVVEIFAKSGKKFSELIDELPKYYQIKTKRHVEG-DRHAIVNKVAEMARERGYTVDTTDGAKIIFEDGWVLVRASGTEPIIRIFSEAKSKEKAQEYLNLGIELL-----

General information: TITO was launched using: RESULT: Template: 1WQA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2773 35133 12.67 80.03 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.74 3D Compatibility (PKB) : 12.67 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_1WQA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WQA-query.scw PDB file : Tito_Scwrl_1WQA.pdb: