Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHKLGLLSSLGLSFISTLTWGEETSTVLETIRIQAESTREDVSQNSSATKFTHDVLDVPFNRAYLSKQIMEQQDVQRIDDALTLVSGVFHQNSFGGGFWDNYSFRGFSTDPNLGAAMIRNGLSVNRG--ISAPKDVVNIESLEFLKGPMAALYGRGETGGLLNLNSKKPQWESESELNLRANTQEQYHISLEHTAPIN--DELAYRLAVAHEDNQSFRDHVSSERWFFSPQLTWKISDQTQLDFDSEFTEHKGTFDRGVSTVNHQ---F----VMDPKTFTGEPDDGDLKIKDYFYQLHLSHEFNPDWKLNSAVSYKDAKMVGFATEPRRM--QAD---------------GRALERQRRYRDYTSEDVLAQTELLGKIDTSW-ARHEILLSTELGQLDYKQNQLRRNHSTSS-TNTID--I---------YQ--PEYGKYLPNLTPFTDT-KERQRYFALNVQDQIFFNDQWSVLLGNRFDQVEQDFKNHIKQTED-NQTLHQNSPRFGVNFKASDQWAFYTNYGRSFAMNSGMNR-NGQTFAPEKGESYEVGTKYKIN-DQSVLSLALFKMKKQNVLTTDPIDKDFQTAAGEVSSKGVEFDLNSQITDRWFINANYSYTDAQIEKDQDLAKGARLSNVPKHQGSVSTNYEFLQDGARKAGVGANLTYVGERSGHNLDNGFNLPSYTLVNLNGYYAPS----DRLRYQLNVNNLFDKTYYVSSYSDLWVQPCEPLNASISAQWKF 1FI1 Chain:A ((8-707)) -------------------------ATIAARQSATG-------------TKTDTPIQKVPQSISVVTAEEMALHQPKSVKEALSYTPGVSVGTRGASNTYDHLIIRGFAAEGQ-SQNNYLNGLKLQGNFYNDAVIDPYMLERAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLDDDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGYYGWLPKEGTVEPLPNGKRLPTDFNEGAKNN-TYSRNEKMVGYSFDHEFNDTFTVRQNLRFAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDTQLQSKF-ATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPSSHHHHHHGSSVNTDFDFNAKDPANSGP-YRILNKQKQTGVYVQDQAQW-DKVLVTLGGRYDWADQESLNRV-AGTTDKRDDKQFTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNI-FAPSKGKQYEVGVKYVPEDRPIVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYTDAEYTTDTT-YKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPA-NSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVVATATFRF

General information: TITO was launched using: RESULT: Template: 1FI1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4017 173813 43.27 267.82 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 43.27 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_1FI1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FI1-query.scw PDB file : Tito_Scwrl_1FI1.pdb: