Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------MRVRRPTMSFAFWS----------RLKALVRKETKQLLRDKGSLGIGLVLPVILILLFGYG---LSFDLNQARVGVVVDQSSPQVNQVLAGLNGSRYLTSL-----EFRNLPEAEQAIRNGKIDAILHLPSDFANQVQQGNA--KVQLLLNGRSTTIATALEG----YVAGALATAPS-----------IQIDRSPILASASAVKIEQRIWFNESGNSTWFLVPGLMILILTLIGAFL-TGLLIARERERGTLEALFVTPVRPFEIVLAKLIPYVVVGMIDIVICIVAAHFIFEVPMRGSLFSILFASFLYLIVSLLL---GLTISGFAQSQFQASQIALLASFMPALMLSGFVFDTRNLPLVVQIISQLLPAT--HFMVLIKTLFMGGDDWKLWFKECGILLGYIVVL-----ICAVNFSLKKRLR- 4B28 Chain:A ((32-468)) RKIDPSRGATLGDGSPNDMNRVEIGPTQLAFAEWHTARLDLPDLAAMRRFRHRRLTDHVVARGYAGLLMFDPLNIRYATDSTNMQLWNTHNPFRATLLCADGY----MVMWDYKNSPFLSEFNPLVREQRAGADLFYFDRGDKVDVAADVFANEVRILLRDHAPGLRRLAVDKVMLHGLRALQAQGFEIMDGEEVTEKARSVKGPDEIRAMRCASHACEVAVRKMEDFARSKVGDGVTCENDIWAILHSENVRRGGEWIETRLLASGPRSNPWFQECGPRVCQRNEIISFDTD---LVGAYGICTDISRSWWIGDQKPRADMIYAMQHGVEHIRTNMEMLKPGVMIPELSANTHVLDAKFQKQKYGCLMHGVGLCDEWPLVAYPDHAVAGAYDYPLEPGMTLCVEALISEEGGDF-----SIKLEDQVLITEDGYENLTKYPFDPALMG

General information: TITO was launched using: RESULT: Template: 4B28.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1833 -65717 -35.85 -178.09 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -35.85 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.227

(partial model without unconserved sides chains): PDB file : Tito_4B28.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4B28-query.scw PDB file : Tito_Scwrl_4B28.pdb: