Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELNLKEDWSKKMKTALNRTPASFPVRRMDYNFEDTPRYWCNHEPSLTHYFTGLSTLFPEGE----SYFVRSVRALRAKAKENEIL-------------DREIGAFIGQEAMHSKEHHAFHVSAQQY----GLNPESLEKATGIV------------------------------LKAIEKVFSKKWNLLVTVGLEHYTAVLVVSMMQSVNELMTENTIRNLWLWHSVEETEHKAVAYDLYQHLYGNGLDAYIPRVGIFTFSLVLITAFSTIYHIVLMKRDKQLTN----FKTWRNFFKFASKQYKVLI-------------PKFLEYYRFDFHPNQ-----TDEKALVAATKVKLGINDRSLLLS-------------------- 3TNF Chain:B ((3-381)) -----LDEYEQAIKRAQENIKKGEELEKKLDKLERQGKDLEDKYKTYEENLEGFEKLLTDSEELSLSEINEKMEAFSKDSEKLTQLMEKHKGDEKTVQSLQREHHDIKAKLANLQVLHDAHTGKKSYVNEKGNPVSSLKDAHLAINKDQEVVEHEGQFYLLQKGQWDAIKNNPAALEKAQKDYSQSKHDLATIKMEALIHKLSLEMEKQLETINNLIMSTDPKENEEATKLLHKHNGLNLKLANLQDMLAVHRKEKSFFNEKGEEVTSLNDAHYVIG--KDQQLFNLGGKFYPIHKEQKILEKDGKFYLLKQGEDWESIKDSPEKQKKAEHDFHKLQYETPMTVKKLVHHNKGLETTIHKERVEETKQQLEDNGKEKIEIANNISK

General information: TITO was launched using: RESULT: Template: 3TNF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 840 30680 36.52 107.27 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 36.52 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.155

(partial model without unconserved sides chains): PDB file : Tito_3TNF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TNF-query.scw PDB file : Tito_Scwrl_3TNF.pdb: