Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKNRFFNTMQIQNQVQLKPFNTLSLDVTASHYTKVKSIEDIEEALAFAK----EHELNVLVLSGGSNMLLPQ-QINALVIHLDIQGIDVLSEDQDFIRVKVGAGQVWHDFVLYTTKQNWFGLQNLALIPGLVGASPVQNIGAYGVEVGEFIESVQVYDRLLKQTGSISAADCHFSYRHSIFKDEP---ARYIITHVTFKLLKQ-ANLKLNYGDLK-Q---AVGDNLTAENLQNQVIHIRQSKLP--DP--KEYPNVGSFFKNPIVNTQEFERLIAQF---STIPHYPQANGNVKIAAGWLIDQAGW-KGKQLGV---VGMFHKQALVLVNYANASLADVKKTYQAVQHDVEQRFQIMLEPEPVLYNNLGLIENHTE 5JZX Chain:A ((44-403)) ----SLFAGAHIAEAVPLAPLTTLRVGPIARRVITCTSAEQVVAALRHLDSAAKTGADRPLVFAGGSNLVIAENLTDLTVVRLANSGITID-----GNLVRAEAGAVFDDVVVRAIEQGLGGLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLDRCTGEVRWVSARDLRFGYRTSVLKHADGLAVPTVVLEVEFALDPSGRSAPLRYGELIAALNATSGERADPQAVREAVLALRARKGMVLDPTDHDTWSVGSFFTNPVVTQDVYERLAGDAATR-PVPHYPAPD-GVKLAAGWLVERAGFGKGYPDAGAAPCRLSTKHALALTNRGGATAEDVVTLARAVRDGVHDVFGITLKPEPVLIGC---------

General information: TITO was launched using: RESULT: Template: 5JZX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1838 -33095 -18.01 -99.38 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -18.01 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_5JZX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JZX-query.scw PDB file : Tito_Scwrl_5JZX.pdb: