Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------MTKFNNNQSDNLFSQVFGV---AKKLSSTGLNILQQSQIGEVSKLVEPLSNG-KTVEGSARNKSPFEVEQYESPLQMLR----EHLPKVTRQVFGRHFRKVNGIATFISPDWNEKISSYLFDWLNDFSSKSTLTEKILEEAGAKDLFELTKDTSRSQRLSQALIEQNKLIASIQGAITGVSGMVGAAVDIPVSLV----LVLRTIYQTGRSHGFDLTEATDQDVVEFIFKE------VDISLIAEKQTLLLALKALRNMLETQDIQ-QFQQVLGSSNDIETLKSWLVDENGKFKWNWLNKVPQLAVV-GKFTPVA--GAVLSAVYSWKLQEDVGHKAQAIFGAARHYLNEHPNE---HLSPLQAYYAA--VTLIQKASPRLLNVGENGSVHATQHHK--------------------IENHDVISKVSVVVKSNTSEKSEEKVQENVHQGIEHLAEKHVVEHEHSEQKPALEPESEENDDVIEGQKYS 5MB9 Chain:A ((23-578)) GERVVIGITFGNSNSSIAHTVDDKAEVIANEDGDRQIPTILSYVDGDEYYGQQAKNFLVRNPKNTVAYFRDILGQDFKSVDPTHNHASAHPQEAGDNVVFTIKDKAEEDAEPSTLTVSEIATRYLRRLVGAASEYLGKKVTSAVITIPTNFT---EKQKAALIAAAAAADLEVLQLISEPAAAVLAYDARPE--ATISDKIIVVADLGGSRSDVTVLASRSGMYTILATVHDYEYHGIALDKVLIDHFSKEFLKKNPGAKDPRENPRSLAKLRLEAESTKRALSRSTNASFSVESLIDGLDFASTINRLRYETIARTVFEGFNRLVESAVKKAGLDPLDVDEVIMSGGTSNT--PRIAANFRYIFPESTRILAPSTDPSALNPSELQARGAALQASLIQEFETEDIEQSTHAAVTTMPHVTNAIGVVSVSKFVPIIAPETAVPARRTVHGGDVLVKVVEGSTHINVIKREKVWKIGSTLAEAAVRGVKKGAKVEVTINVNTDLTVIVTAREV

General information: TITO was launched using: RESULT: Template: 5MB9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2423 148428 61.26 342.00 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 61.26 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.157

(partial model without unconserved sides chains): PDB file : Tito_5MB9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MB9-query.scw PDB file : Tito_Scwrl_5MB9.pdb: