Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKTSVIYPGTFDPITNGHVDLVTRASRMFDEVVVAIAIGHHKNPLFSLEERVALAQSSLGHLSNVEFVGFDGLLVNFFKEQKATAVLRGLRAVSDFEYEFQLANMNRQLDPHFEAVFLTPSEQYSFISSTLIREIARLKGDVTKFVPQAVVEAFERKHQQGW 5TS2 Chain:D ((12-165)) -----VLYPGTFDPITKGHGDLIERASRLFDHVIIAVAASPKKNPLFSLEQRVALAQEVTKHLPNVEVVGFSTLLAHFVKEQKANVFLRGLRAVSDFEYEFQLANMNRQLAPDVESMFLTPSEKYSFISSTLVREIAALGGDISKFVHPAVADALAERF----

General information: TITO was launched using: RESULT: Template: 5TS2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 731 -117127 -160.23 -760.56 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain D : 0.91 3D Compatibility (PKB) : -160.23 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.621

(partial model without unconserved sides chains): PDB file : Tito_5TS2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TS2-query.scw PDB file : Tito_Scwrl_5TS2.pdb: