Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAALLGVNIDHVATLRQARGTAYPDPVEAALICEQAGAEGITLHLREDRRHIQDDDVRRMRPLLKTRMNLEMAVTDEMVEFAKEIQPQHVCFVPERRQEVTTEGGLDVVGNFEKVKAATQALAAIGCDVSLFIDADFAQIDAAIACGAPTIELHTGAYTDATTEQEQQEELARIVKGVEYAASKGLVVNAGHGLNLKNIAPIAAIPQIHALNIGHSIIAESVFVGLVQAVKDMKTAIQAAG 1HO4 Chain:C ((4-234)) ---LLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVTTEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYADAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAIIGRAVMTGLKDAVAEMK-------

General information: TITO was launched using: RESULT: Template: 1HO4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1340 -173828 -129.72 -752.50 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain C : 0.89 3D Compatibility (PKB) : -129.72 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_1HO4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HO4-query.scw PDB file : Tito_Scwrl_1HO4.pdb: