Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIKSDRWIREMSE-KHGMIEPYAENQVRFDKNGEKLISYGVSSYGYDVRCAREFKVFTNVHSAIVDPKNFDE--KSFIDI-ESDVCIIPPNSFALARTIEYFRIPRNVLTVCLGKSTYARCGIIVNV--TPLEPEWEGHVTLEFSNTTNLPARIYAGEGVAQMLFFESDEVCETSYKDR--GGKYQGQTGVTLPKT 2QXX Chain:B ((1-181)) -MLLSDRDLRAEISSGRLGIDPFDDTLVQP--------------SSIDVRLDCLFRVFNNTRYTHIDPAKQQDELTSLVQPVDGEPFVLHPGEFVLGSTLELFTLPDNLAGRLEGKSSLGRLGLLTHSTAGFIDPGFSGHITLELSNVANLPITLWPGMKIGQLCMLRLTSPSEHPYGSSRAGSKYQGQRGPTPSR-

General information: TITO was launched using: RESULT: Template: 2QXX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 783 11550 14.75 66.76 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 14.75 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_2QXX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QXX-query.scw PDB file : Tito_Scwrl_2QXX.pdb: