Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVIALFSKNWSRTMSATIVNTTDDNFQADVLDAETPVLVDFWAGWCAPCKAIAPVLEDLSSEYAGKVKIVKVDVTSCEETAVKYNIRNIPALLLFKNGEVVAQQIGAVPRSKLVSFIDENV 2FCH Chain:E ((2-107)) ---------------DKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRSIPTLLLFKNGEVAATKVGALSKGQLKEFLDANL

General information: TITO was launched using: RESULT: Template: 2FCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 464 -83240 -179.40 -785.28 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain E : 0.82 3D Compatibility (PKB) : -179.40 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.833

(partial model without unconserved sides chains): PDB file : Tito_2FCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FCH-query.scw PDB file : Tito_Scwrl_2FCH.pdb: