Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAVNFNMRLEAELKEQVTPILEDYGLTMPQAFKLFLNQIVKTKAIPLSFDYARETALTPKAAAKLLQSLKEIENGEY-TEYETVEEAIKAMSEEAHG 4Q2U Chain:I ((3-85)) -ANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLRE--PNQLT---IQ----SIKNSEAGIDVHKAKDADDLFDKLG-----

General information: TITO was launched using: RESULT: Template: 4Q2U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 91 -10481 -115.18 -127.82 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain I : 0.76 3D Compatibility (PKB) : -115.18 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_4Q2U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q2U-query.scw PDB file : Tito_Scwrl_4Q2U.pdb: