Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKGAIFLRPKQQLFITLLVILGALFNN--------ISVSASAFNFSVTPITSENQIDKRKTYF----DLQLDPDQEVEVKAELRN--DTEKEVKIDISVNSATTNSNVMVEYGKNEIEKDMS-----LIFDLVDYVSYP-----QIVTLKPKSVQTVAFHVRMP--------NERFDGVLAGGITFQE-IQTEKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQNDQKTYINQVVIETKITKKGHSEVLYQ----------------EEKEGLQIAPNTNFSFP------------------TALNGQPLTPGEYHLTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNNWIYILVASLFLLFILLCILLFVLRKKEEKEK 2VSM Chain:A ((1-416)) -----ICLQKTSNQILKPKLISYTLGQSGTCITDPLLAMDEGYFAYSHLERIGSCSRGVSKQRIIGVGEVLDRGDEVPSLFMTNVWTPPNPNTVYHCSAVYNNEFYYVLCAVSTVGDPILNSTYWSGSLMMTRLAVKPKSNGGGYNQHQLALRSIEKGRYDKVMPYGPSGIKQGDTLYFPAVGFLVRTEFKYNDSNCPITKCQYSKPENCRL----SMGIRPNSHYILRSGLLKYNLSDGENPKVVFIEISDQR----LSIGSPSKIYDSLGQPVFYQASFSWDTMIKFGDVLTVNPLVVNWRNNTVISRPGQSQCPRFNTCPEICWEGVYNDAFLIDRINWISAGVFLDSNQT------AENPVFTVFKDNEILYRAQLASEDTNAQKTITNCFLLKNKIWCISLVEIYDTGDNVIRPKLFAVKIPEQCTH

General information: TITO was launched using: RESULT: Template: 2VSM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1914 52421 27.39 151.51 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 27.39 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.193

(partial model without unconserved sides chains): PDB file : Tito_2VSM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VSM-query.scw PDB file : Tito_Scwrl_2VSM.pdb: