Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVKESRNSY-GYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGT------------VGKIAKNVLKRNFSVDTP-NKKWVTDVTEFKIKGRKIYLSPYT 4IQJ Chain:M ((1-177)) HHHHHHKAGEAQDLAEGWRAFL------EALKPTLRAFVREARPHLEGKTLVLRFPESKAFHHLLPLARAQFGVEELAFVLEKKSLSLEDPPWEAEEGEDPSEELRRLARLLGGRLLWVRKPKAPEAEEPVSEDGIGGNGIMPP---

General information: TITO was launched using: RESULT: Template: 4IQJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 300 3158 10.53 25.47 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain M : 0.64 3D Compatibility (PKB) : 10.53 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.057

(partial model without unconserved sides chains): PDB file : Tito_4IQJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IQJ-query.scw PDB file : Tito_Scwrl_4IQJ.pdb: