Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGTEQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQ-IIHAYPHGGGAYVVSSENLGKNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLRGLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILRAFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMM-ALILGFFFVGITFINYWYGIVPEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMPHMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVVKWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDHYKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDEDVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIPQFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK 5DA0 Chain:A ((14-484)) ---------QREWFANPRKDVLAGIVVALALIPEAIAFSIIAG---------------VDPQVGLYASFIIALITAFLGGRPGMISAAT---GAMALLMTGLVKDHG--------IQYLFAATVLTGVLQVVFGWAKLARYLKFVPRSVMVGFVNALAILIFMAQLPQFVGANWQMYAMVAAGLAIIYL-------LPLVFKAMPSALVAIVVLTVVAVVTGADVKTVGDMGTLPTALPHFQFPQVPLTFETLAIIFPVALTLSLVGLLESLLTAQLIDERTDTT-SDKNVESRGQGVANIVTGFFGGM--AGCAMIGQSMINVTS--------------------GGRGRLSTFVAGAFLMVLILA-----LQPLL---VQIPMAALVAVMMVVAISTFD-WGSLRFPKGETVVMLATVAVTVFTHD----------LSLGVLIGVVLSALFFARKVS-QLSQVTPVDEVDGTRTYRVRGQLFFVS---------------------------THDFLHQFDFTHPARRVVIDLSDAHFWDGSAVGALDKVMLKFMRQG--TSVELRGL-----------NAASATLVERL-----------------

General information: TITO was launched using: RESULT: Template: 5DA0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2470 -300622 -121.71 -646.50 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -121.71 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.261

(partial model without unconserved sides chains): PDB file : Tito_5DA0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DA0-query.scw PDB file : Tito_Scwrl_5DA0.pdb: