Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYLQDIDQQNMEGRKMKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDMPAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALLAYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLWYARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRSGKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELAGQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFLNEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYAAGGIVIKHK--NMRNIISYFAPYVFEKSVK--APDMLGAYILEGSKAGATAAAVWTAHRVLPLNVTGYGQLIGASIEAAQRFREFLDHL-TFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC-------TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEEWKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ 2JIS Chain:A ((83-514)) ------------------------------------------------------------------------RSQGESQKQILERCRAVIR-YSVKTG-HPRFFNQLFSGLDPHALAGRIITESLNTSQYTYEIAPVFVLMEEEVLRKLRALVGWSSGDGIFCPGGSISNMYAVNLARYQRYPDCK-Q--------------------------------------------RGLRTLPPLALFTSKECHYSIQKGAAFLGLGTDSVRVVKADERGKMVPEDLERQIGMAEAEGAVPFLVSATSGTTVLGAFDPLEAIADVCQR-----HGLWLHVDAAWGGSVLLS-------------------------------QTHRHLLDGIQRADSVAWNPHKLLAAGLQCSALLLQDTSNLLKRCHGS---------KFYDVALDTGDKVVQCGRR-VDCLKLWLMWKAQGD--QGLERRIDQAFVLARYLVEEMKKREGFE-------LV--MEPEFVNVCFWFVPPSLRGKQESPDYHERLSKVAPVLKERMVKE------GSMMIGYQPHG-------------------------TRGNFFRVVVANSALTCADM-----DFLLNELERLGQD---

General information: TITO was launched using: RESULT: Template: 2JIS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2212 -169783 -76.76 -413.10 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -76.76 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_2JIS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JIS-query.scw PDB file : Tito_Scwrl_2JIS.pdb: