Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNYYGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVIPQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDVQTLTDNSIKEVDKIAADKEKELLEV 1EH1 Chain:A ((2-185)) TLKELYAETRSHMQKSLEVLEHNLAGLRTGRANPALLLHLKVEYYGAHVPLNQIATVTAPDPRTLVVQSWDQNALKAIEKAIRDSDLGLNPSNKGDALYINIPPLTEERRKDLVRAVRQYAEEGRVAIRNIRREALDKLKKLAKELHLSEDETKRAEAEIQKITDEFIAKADQLAEKKEQEILG-

General information: TITO was launched using: RESULT: Template: 1EH1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 683 -87779 -128.52 -477.06 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -128.52 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.627

(partial model without unconserved sides chains): PDB file : Tito_1EH1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EH1-query.scw PDB file : Tito_Scwrl_1EH1.pdb: