Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSSSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKQFHLRYLSRLKRNSRSSIATNRVFQMPLRCPTQTASLRG 1OTH Chain:A ((1-321)) KVQLKGRDLLTLKN----FTGEEIKYMLWLSADLKFRIKQKGEYLPLLQGKSLGMIFEKRSTRTRLSTETGFALL------GGHPCFLTTQDIHLGVNESLTDTARVLSSMADAVLARVYKQSDLDTLAKEA-----S-IPIINGLSD-LYHPIQILADYLTLQEHYS--SLKGLTLSWIGDG-NNILHSIMMSAAKF-GMHLQAATPKGYEPD------------ASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMG-REEEKKKRLQAFQGYQVT------------MKTAKVA-ASDWTFLHCLPRK-P-------------------EEVDDEVFYSPRSLVFPEAENRKWTIMAVMVSLLTDYSPQLQKPKF--

General information: TITO was launched using: RESULT: Template: 1OTH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1869 -8925 -4.78 -27.89 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -4.78 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_1OTH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OTH-query.scw PDB file : Tito_Scwrl_1OTH.pdb: