Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLYKPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPECSVDHRSFPHRPTYWQNFLESSSERNHGTT 2LVR Chain:A ((1-30)) -------------------------------------------------------------------------------------MKPYVCIHCQRQF-ADPGALQRHVRIHTGEK----------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2LVR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 59 -9475 -160.59 -315.83 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.50 3D Compatibility (PKB) : -160.59 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_2LVR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LVR-query.scw PDB file : Tito_Scwrl_2LVR.pdb: