Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIV-----DAVFSPAP-HTC-RN--CGSTVVDGNGK-----VIVVKNGKKETIVRFEQYNHMPLVM----------RLKKQRYTCKNCRTH---WTT-QSYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKE-FKSYLPKQSKRILPRVLMVDE------------------FRSHASIEDKMSFICADGETGQLIDV------LPTRKLP----RLTSYFLGCANPEEVKFLVTD-M-------NAAYFQLTKRILPNAKVVIDRFHIIKH------MNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAM-MIDRLLGFSTSLKEAYPYFHELV-EAFRDKDPDLFFSLLA------ELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNT--HIKTMKRVSYGFKSFENMRIRIF-LINQLIKVR------------------------------------------------------------------------------------------------------------------------------ 2ID0 Chain:A ((5-644)) -NPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSYFIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPDHPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVPWWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALPDDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRANEVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIAEQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEAMIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFCKLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSP--IRKYGDMINHRLLKAVIKGRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAAEIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVIDVTIAEVRMETRSIIARPVA

General information: TITO was launched using: RESULT: Template: 2ID0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1763 30087 17.07 70.30 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 17.07 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_2ID0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ID0-query.scw PDB file : Tito_Scwrl_2ID0.pdb: