Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKSGTTRKASFDKETYLKGASFLYKKMISYIKFLRKEWKVMSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY-QHR----LFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHGVTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAVGVPAKVVRIKGEKVRRSE 1SST Chain:C ((81-240)) ------------------------------------------------IQAVRHRDPAVELWSTPLLYLKGFHAIQSYRITHYLWNQNRKSLALYLQNQISVAF-----DVDIHPAAKIGHGIMFDHATGIVVGETSVIENDVSILQGVTLGGTG----DRHPKVREGVMIGAGAKILGNIEVGKYAKIGANSVVLNPVPEYATAAGVPARIV-----------

General information: TITO was launched using: RESULT: Template: 1SST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 826 -19557 -23.68 -129.52 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -23.68 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_1SST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SST-query.scw PDB file : Tito_Scwrl_1SST.pdb: