Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAISTVNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAENSEYIQIKTINDK- 1S12 Chain:A ((1-94)) MIKVTVTNS------FFEVTGHAP-----DKTLCASVSLLTQHVANFLKA----EKKAKIKKESG---YLKVKF---EELENCEVKVLAAMVRSLKELEQKFPSQIRVEVIDNGS

General information: TITO was launched using: RESULT: Template: 1S12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 358 -47072 -131.49 -506.15 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -131.49 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_1S12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S12-query.scw PDB file : Tito_Scwrl_1S12.pdb: