Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAPPSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRNIFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN 4UC2 Chain:A ((11-149)) ------------LAACGAPATTGALL--KPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRVAQLEG---SGQALAFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPYLIWATATTGLNFEAMRLN

General information: TITO was launched using: RESULT: Template: 4UC2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 544 -122467 -225.12 -881.05 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -225.12 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_4UC2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UC2-query.scw PDB file : Tito_Scwrl_4UC2.pdb: