Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGINIENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDP-ISPCGACRQVMAEFCSPDMPVTLVGDNGVTKATTVRALLPYAFTEKDL 2FR6 Chain:C ((16-142)) -----QRLLLSSREAKKSAYCPYSRFPVGAALLTGDGRIFSGCNIENACYPLGVCAERTAIQKAISEGYKDFRAIAISSDLQEEFISPCGACRQVMREF-GTDWAVYMTKPDGTFVVRTVQELLPASFGPEDL

General information: TITO was launched using: RESULT: Template: 2FR6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 630 -38453 -61.04 -305.18 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -61.04 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2FR6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FR6-query.scw PDB file : Tito_Scwrl_2FR6.pdb: