Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQKRQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALGVDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGLKVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGP-------------REWYDDEFEVYGHYMPLDELLDQVDVMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADSLVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA 5G1P Chain:D ((56-311)) --------------------------------------------------SMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSV---ESLADSVQTMSCYA-DVVVLRHPQPGAVE--LAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSMPPTVRAFVASRG-KQEEFE------SIEEALPDTDVLYMTRIQKER---------EACFGQFILTPHIMTRAKKKMVVMHPMP--RVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVL----

General information: TITO was launched using: RESULT: Template: 5G1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1116 -67983 -60.92 -295.58 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : -60.92 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_5G1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G1P-query.scw PDB file : Tito_Scwrl_5G1P.pdb: