Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREYKGLKIAVAGTGYVGLSIATLLAQ---SHTVTAVDIIPEKVELINNKKSPIQDDYIEMYLAK-KELDLMATVDGVSAYKDADFVVIAAPTNYDSK----KNFFDTSAVEAVIKLVMEV-NPEAIMVIKSTIPVGYTASIREKT-----GSKNIIFSPEFLRESKALYDNLYPSRIIVGTDMEDERLVEAAQTFAKLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQQIINGVCRDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYE--DVP--ENLIEAIVESNRTRKNFIADRVLELAGAYEANSHWDASKEKKVVIGVYRLTMKSNSDNFRQSSIQGVMKRVKAKGTTVVIYEPSLKDGEIFF-------------GSKVVNDLDQFKAMCDSIIANRYDTCLDDIK---------E---KVYTRDIFQRD 2O3J Chain:C ((6-452)) --FGKVSKVVCVGAGYVGGPTCAMIAHKCPHITVTVVDMNTAKIAEWNSDKLPIYEPGLDEIVFAARGRNLFFSSDIPKAIAEADLIFISVNTPTKMYGRGKGMAPDLKYVESVSRTIAQYAGGPKIVVEKSTVPV-AAESIGCILREAQKLKFQVLSNPEFLAEGTAMKDLANPDRVLIGGESS-PEGLQAVAELVRIYENWV-P-RNRIITTNTWSSELSKLVANAFLAQRISSINSISAVCEATGAEISEVAHAVGYDTRIGSKFLQASVGFGGSCFQKDVLSLVYLCESLNLPQVADYWQGVININNWQRRRFADKIIAELFN----------TVTDKKIAIFGFAFKKNTGDTRESSAIHVIKHLMEEHAKLSVYDPKVQKSQMLNDLASVTSAQDVERLITVESDPYAAARGAHAIVVLTEWDEFVELNYSQIHNDMQHPAAIFDGRLILDQ-

General information: TITO was launched using: RESULT: Template: 2O3J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1990 319 0.16 0.80 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : 0.16 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_2O3J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O3J-query.scw PDB file : Tito_Scwrl_2O3J.pdb: