Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRMIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARCPYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILPGTRAAVK--KTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEYHSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGEVSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK 3UHO Chain:B ((27-271)) -------------IGVFDSGVGGLSVLKSLYEARLFDEIIYYGDTARVPYGVKDKDTIIKFCLEALDFFEQFQIDMLIIACNTASAYALDALRAKAHFPVYGVIDAGVEATIKALHDKNKEILVIATKATIKSEEYQKRLLSQ-GYTNINALATGLFVPMVEEGIFEGDFLQSAMEYYFKNITTP--DALILACTHFPLLGRSLSKYFGDKTKLIHSGDAI---VEFLKERENI--DLKNHKAKLHFYASSDVESLKNTAKIWLNL-------------

General information: TITO was launched using: RESULT: Template: 3UHO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1402 -84549 -60.31 -347.94 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -60.31 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_3UHO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UHO-query.scw PDB file : Tito_Scwrl_3UHO.pdb: