Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGARRVPKYFPSMA 4ZFM Chain:D ((30-198)) -------QVEGAWNEDGKGLSVWDV-------FAKQPGRTFKGT--------NGDVAVDHYHRYQEDVALMAEMGLKAYRFSVSWSRVFPDGN-GAVNEKGLDFYDRLIEELRNHGIEPIVTLYHWDVPQALMDAYGAWESRRIIDDFDRYAVTLFQRFGDRVKYWVTLNQQNIFISF------------------------

General information: TITO was launched using: RESULT: Template: 4ZFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 659 -65991 -100.14 -425.75 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -100.14 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.588

(partial model without unconserved sides chains): PDB file : Tito_4ZFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZFM-query.scw PDB file : Tito_Scwrl_4ZFM.pdb: