Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAK-NEYGVIYVTEQCAALSPETIERYK-EQMIPAIVLIPNHQGS-LGIG-LEEIQKNVEKAVGQNIL 2D00 Chain:B ((3-107)) -PVRMAVIADPETAQGFRLAGLEGYGASSAEEAQSLLETLVERGGYALVAVDEALLPDPERAVERLMRGRDLPVLLPIAGLKEAFQGHDVEGYMRELVRKTIGFDI-

General information: TITO was launched using: RESULT: Template: 2D00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 273 -22440 -82.20 -222.18 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -82.20 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_2D00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2D00-query.scw PDB file : Tito_Scwrl_2D00.pdb: