Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence FGRRRDGRMPPWEWVTWKDFCRLTLKKGDEIQSQSKWVAKPGPIGILIQPGVYESFKTDKDCNPKIRRGKLPDGYTLHGQLKYITP-DLASMYNRVLDDT-KWWPFDSQEAPQTSTTVEKSL------------ 1JSP Chain:B ((1-121)) -GSHMRKKIFKPE--ELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLS-TIKR-KLDTG-------QYQEPWQYVDDVWLMFNNAWLYNRKTSRVY-KFCSKLAEVFEQEIDPVMQSLG

General information: TITO was launched using: RESULT: Template: 1JSP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 374 172 0.46 1.61 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 0.46 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_1JSP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JSP-query.scw PDB file : Tito_Scwrl_1JSP.pdb: