Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNA--KSKGVKDGIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEITDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYTGPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEG------EREFGATVIDMGAGQTTVATIRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEVIGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRL---LDLPGGIVLIGGNAILPGMVELAQEVFGVRVKLYVPNQV------------GIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSHQPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGLIGSMFDE 5EQ6 Chain:A ((11-355)) --KANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLV----KAKTNLKSAVVAVAGSAVITKTIE--MEA-----GLSEDELENQLKIEADQYIP-------YPLEEVAID-FEVQGLSA---RNPERVDVLLAACRKENVEVREAALALAGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSVEEAGLAKKQGGLPDD------------------------YDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANPFADMALNGKVNAGALASDAPALMIACGLALRS---------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EQ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1490 -6769 -4.54 -21.02 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -4.54 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_5EQ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EQ6-query.scw PDB file : Tito_Scwrl_5EQ6.pdb: