Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALIVQKYGGTSMGTPERILNVARRVKRWHDHGHKVVVVVSAMSGETNRLLALA------------------------KAITETPDPREI---------------------------------DQMVSTGEQVTISMLAMALNSIGVEAKSYTGRQVGIKTDSAFTKARIESIDT-DVMTNDLDAGRVIVVAGFQGFDANGNTTTLGRGGSDTSGVAIAAALKADECQIYTDVDGVYTTDPRVAPKAKKIDRISFEEMLEMASLGSKVLQIRSVEFAGKYQVPLRVLSSFDNDDDGAFDDEFKENVGTLITTEAEDTMEQPIIAGIAFNRDEAKLTIL--GVPDEPGIASKILSPVSDANIEVDMIVQNVEEDGTTDFTFTVNRVDLAKAEKILNETAKNIGA---------REVSTRDDIVKVSIVGVGMRSHAGVASKMFTALADEGINILMIS--TSEIKVSVIIDEKYLELAVRCLHTAFGLDREHGESSARA 3C20 Chain:B ((4-467)) ----VMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVRDIAKVGDFIKFIREKHYKAIEEAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPKSRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDPRLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESE-----------GTLITNDME--MSDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSE---TNISLVVSEEDVDKALKALKREFGD---KSFLNNNLIRDVSVDKDVCVISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKF-------------

General information: TITO was launched using: RESULT: Template: 3C20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2103 -7286 -3.46 -18.68 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -3.46 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_3C20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C20-query.scw PDB file : Tito_Scwrl_3C20.pdb: