Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKGPDMQAIILDTETHTLNGL---PIEIAYAPIEINAGKLTLDKSKLFDQLYQV--GTPISYAAMAVHHILESD-----LENQPHYKTFKL----PDT-----TTYIIGHNIDYDIAAIARC----GVD---VSHIKPICTLALTRKTWPDAEAHNISALIYMISQGSSKARELLKGAHRADADIILTANILMHIVYHLNIHDIEELYRVSEEARIPTTINFGKHKGTAIAELPKDYIQWLLRQDELDVYLRKALESAF 2F96 Chain:B ((27-214)) ----RGYLPVVVDVETGGFNSATDALLEIAATTVGMDEKG-FLFPEHTYFFRIEPFEGANIEPAALEFTGIKLDHPLRMAVQEEAALTEIFRGIRKALKANGCKRAILVGHNSSFDLGFLNAAVARTGIKRNPFHPFSSFDTATLAGLAYG---QTVLAKACQAAGMEFD-----NREAHSARYDTEKTAELFCGIVNRWK-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2F96.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 717 -45311 -63.20 -279.70 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -63.20 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_2F96.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F96-query.scw PDB file : Tito_Scwrl_2F96.pdb: