Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKLIITAAVNGGITPRSKNPAVPYTPEEIANAVYEVWNAGASVAHIHARNLD-GSPSYQQEVWGEIVDKVRARCDIILNLSTSGLN--LPLDAPKDQAWNHLVY-RPEIASYNCGSVNHGS------------------------KPFINPPALAMELADAINQYGVKPEIEIYHSGVINEAETLHLKGYLKSPMLFAFAMGIHGGVTATCKNLIHLID----SLPA-GSLWSALGIGKAQLPINVHTILLGGHVRTGLEDNIYYKAGELATSNAQLVERLVRLSHELDRPVASTQEARKILGLVS 3LOT Chain:D ((5-308)) DDVVIVTCAITGAIHTPSMSPYLPVTPDQIVEEAVKAAEAGAGMVHIHARDPKDGRPTTDVEVFRYICREIKKQSDVVINVTTGGGGTLGIP--V-EERAKVVPALKPEIATFNMGSMNFAIHPLLKKYKEFKYDWEPEYLEMTRDIVFRNTFKDLEALSRIFKENDTKPELECYDIGQIYNTAFMFHEGYLEPPLRLQFIHGILGGIGTAVEDVLFMKQTADRLIGRENYTWSLVGAGRFQMPLGTLAVIMGGDVRVGLEDSLYIERGKLAKSNAEQVEKMVRIVKELGKRPATPDEVREILGLKG

General information: TITO was launched using: RESULT: Template: 3LOT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1575 -53948 -34.25 -199.07 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.77 3D Compatibility (PKB) : -34.25 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_3LOT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LOT-query.scw PDB file : Tito_Scwrl_3LOT.pdb: