Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MLRLKCLGGPLYGQEYSHAGDEFLFNDKQSGK-QTLYRKQTLDFTPPQDVFVAESISNIIAYNLALQLKK-------- 5I5H Chain:A ((246-572)) YPLSELRYRDMGTGQNVLLITVDGLNYSRFEKQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITALNQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNRWFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAGRGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP--ASEYSQGQD--LFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDEKPQLSL

General information: TITO was launched using: RESULT: Template: 5I5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 82 3315 40.42 50.99 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 40.42 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_5I5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I5H-query.scw PDB file : Tito_Scwrl_5I5H.pdb: