Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILNAEQSMVQEMMRNYAQNQLKPTAAHRDKTHEFPAQELKDLGALGAMGMTVPDEWGGAGMDYVSLVLAIEEIAAGDGAISTIVSVQNSLICGITLAYGSEQQKQTYLPKFASGEWLGCFCLTEPHVGSDASAILCKAERDGDHWILNGVKQFITSGKNAQVALVFAVTDKQAGKKGISCFLVPTNTQGYLVTRIEDKMGQHASDTATITLEDCRIPLENLVGQEGEGYKIALSNLAAGRIGIAAQSVGMARAAFDAAVQYANERKAFGVELVQHQAVGFRLADMATQIEAAHQLVLHAATLKDAGLPCLKEASMAKLFASTMAERVCSDAIQIHGGYGYVSDFPVERIYRDVRVSQIYEGASDIQRLVIAREVAQV 2VIG Chain:B ((6-375)) --LPETHQMLLQTCRDFAEKELFPIAAQVDKEHLFPAAQVKKMGGLGLLAMDVPEELGGAGLDYLAYAIAMEEISRGCASTGVIMSVNNSLYLGPILKFGSKEQKQAWVTPFTSGDKIGCFALSEPGNGSDAGAASTTARAEGDSWVLNGTKAWITNAWEASAAVVFAST-------SISAFLVPMPTPGLTLGKKEDKLGIRGSSTANLIFEDCRIPKDSILGEPGMGFKIAMQTLDMGRIGIASQALGIAQTALDCAVNYAENRMAFGAPLTKLQVIQFKLADMALALESARLLTWRAAMLKDNKKPFIKEAAMAKLAASEAATAISHQAIQILGGMGYVTEMPAERHYRDARITEIYEGTSEIQRLVIA------

General information: TITO was launched using: RESULT: Template: 2VIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2214 -209501 -94.63 -577.14 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -94.63 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_2VIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VIG-query.scw PDB file : Tito_Scwrl_2VIG.pdb: