Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MRKLVSIII---LLGIAWVAKLSYDMWQISRTVPELQQS---LLQ-----SEQQYANLNDQLVALRRQIQNQPSHNSKT-------TPLATTEVVQTGIAPTVLIKQKLELIQFAIDQQQFIYAVDHLTQLQQTLPQYGIAPALQHSLNQALEQDKQA--IQQFVLAQNQRHQLIDDLL-------QNIDKNIQQALKQPKFEMDQSEAVSWWKKWFRIEKVETPSI--------------NLMNRSVVLK----------EVQLRLLIAEQALNQGKMAEYQNELQTVMQKLNELPDATSQQLKNRIAKVAHLSVVPVPKLSTLGLIGS 1GEH Chain:A ((136-443)) FDGPAFGIEGVRKMLEIK-DRPIYGVVPKPKVGYSPEEFEKLAYDLLSNGADYMKDDENLTSPWYNRFEERAEIMAKIIDKVENETGEKKTWFANITA-DLLEMEQRLEVLADL-----GLKHAMVD--VVITGWGALRYIRDLAADYGLAIHGHRAMHAAFTRNP-YHGISMFVLAKLYRLIGIDQLHVGTAEGGKWDVIQNARILRESHYKPDENDVFHLEQKFYSIKAA-FPTSSGGLHPGNIQPVIEALG-TDIVLQLGGGTLGHPDGPAAGARAVRQAIDAIMQ-----------GIPLDEYAKTHKELARALEKWGHVTP--------------

General information: TITO was launched using: RESULT: Template: 1GEH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1200 22777 18.98 94.12 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 18.98 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_1GEH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GEH-query.scw PDB file : Tito_Scwrl_1GEH.pdb: