Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFQVSTLSQKNHVIIQITDTHLLEYPQLEF-----------------------VGMNPEESFHAIIQQILKQ-HPEADAIIHTGDLAQAPTPIT---------YKRYINFMQTL--GLPFFQTLGNHDNVDHFP-----------------LHNENH-------QQPVVVGLG----NWRVIMLNSAVKGK-----VDGHLSSEQLENLANLLEQF--ADHPVLLACHHHPFAMKSKWI-DHHKLQNSNALLDTLSPFQN-VKALVCGHVHQDSLNIWQG------VEFFSTPSTSVQFKPFSNDFALDQNAPGYRYIRLNNDGSFETKVFRLENFKTRINTDISGY 5KAR Chain:A ((12-322)) -----------LGRFWHISDLHLDPNYTVSKDPLQVCPSAGSQPVLNAGPWGDYLCDSPWALINSSLYAMK-EIEPKPDFILWTGDDTPHVPNESLGEAAVLAIVERLTNLIKEVFPDTKVYAALGNHDFHPKNQFPAQSNRIYNQVAELWRPWLSNESYALFKRGAFYSEKLPGPSRAGRVVVLNTNLYYSNNEQTAGMADPGEQFRWLGDVLSNASRDGEMVYVIGHVPPGFFEKTQNKAWFRESFNEEYLKVIQKHHRVIAGQFFGHHHTDSFRMFYDNTGAPINVMFLTPGVTPWKTTLP-GVVDGANNPGIRIFEYDRA------------------------

General information: TITO was launched using: RESULT: Template: 5KAR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1133 59 0.05 0.25 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 0.05 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_5KAR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KAR-query.scw PDB file : Tito_Scwrl_5KAR.pdb: