Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIAKLKIAIIGLGYVGLPLAVEFGK---KVPVVGFDIYQKRIDELKNGQDHTLEVSPEELKQ-A--VHLKYTAH-LDDLQDSNFFIVTVPTPIDDF-----KQPDLTPLIKASTSIGQVLKKGDVVVYESTVYPGATEEVCIPVLEKISGLKFNQDFFAGYSPERINPGDKQHRVTNILKIT-SGST-PEVADYI---DEVYNLIIEAGTHKAPNIKVAEAAKVIENTQRDVNIALINELALIFNKLDIDTEDVLKAAGTK--WNFLPFRPGL-VGGHCIGVDPYYLTHKAQSIGLHP--EIILAARRLNDRMGEHVATQLIKEMVKQRIQVVGARILVMGLSFKENCPDIRNTKIVDFIKALKEYDLDLDIYDPWVDDTEVQHE-------------YGLAPIKELQQGLYDAIVIAVAHNQFKSMSVEEFHALGKEKHVLYDLKYVLDKESSNIRL 2O3J Chain:A ((5-455)) VFGKVSKVVCVGAGYVGGPTCAMIAHKCPHITVTVVDMNTAKIAEWNSDKLPIYEPGLDEIVFAARGRNLFFSSDIPKAIAEADLIFISVNTPTKMYGRGKGMAPDLKYVESVSRTIAQYAGGPKIVVEKSTVPVKAAESIGC-ILREAQK----LKFQVLSNPEFLAEGTAMKDLANPDRVLIGGESSPEGLQAVAELVRIYENWVPRNRIITTNTWSSELSKLVANAFLAQRISSINSISAVCEATGAEISEVAHAVGYDTRIGSKFLQASVGFGGSCFQKDVLSLVYLCESLNLPQVADYWQGVININNWQRRRFADKIIAELFN---TVTDKKIAIFGFAFKKNTGDTRESSAIHVIKHLMEEHAKLSVYDPKVQKSQMLNDLASVTSAQDVERLITVESDPYAAARGAHAIVVLTEWDEFVELNYSQIHNDMQHPAAIFDGRLILDQKAL----

General information: TITO was launched using: RESULT: Template: 2O3J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2178 5976 2.74 14.50 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 2.74 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_2O3J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O3J-query.scw PDB file : Tito_Scwrl_2O3J.pdb: