Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDYQNAVQAFDLESTAKQMLSGSLDALNACYECCDRH--ADGDKIALYWQGKDGRKEQY-TFRELKEWSSQFANFLKSQGVKAGDRISGLLPRTPELIVTILAAWRIGAVYQPLFTAFGPKAIEHRIQLAQSKLVVT-DMGNRS--------KLDEIEKCPAIMTVADAQGTPLKAGDFNF-------WNE-VKQQSDQCDLVMRSIQDPFLLMFTSGTTG-PAKPLEVPLKALIAFGRYMQDAIGLTEEDSFWNIADPGWAYGLYYAITGPLFLGHATLFYEGGFSIDS---LCQIVKDYKVNNLAGAPTAYRMMMA-ADPAQMAPLKGQFRVVSSAGEPLNPEVIRWFKQVL---DAPIYDHYGQTEVGMVVCNHHGLKHEIHAGSAGFPSPGYRVAIVNEQGEELPPDTPGILAVDIS---QSPMMWFGGYKESRKSPF--IGHYYLTGDTAELHADGSMSFVGRSDDVITTSGYRIGPFDVESALLEHDAVIEAAVIGVPDPDRTEVVKAFVILAAGIQPSDALAEELSQFVKRRLSAHAYPRLVEFVSELPKTPSGKIQRFLLRNQEIAKQQAKAG 1PG4 Chain:B ((75-620)) ---------------------------LNLAANCLDRHLQENGDRTAIIWEGDDTSQSKHISYRELHRDVCRFANTLLDLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGCIIDSSSRLVITADEGVRAGRSIPLKKNVDDALKNPNVTSVEHVIVLKRTGSDIDWQEGRDLWWRDLIEKASPEHQPEAMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAATTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMCQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGKEKCPVVDTWWQTETGGFMITPLPGAIELKAGSATRPFFGVQPALVDNEGHPQEGATEGNLVITDSWPGQARTL-FGDHERFEQTYFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHAIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILR------------

General information: TITO was launched using: RESULT: Template: 1PG4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3121 -46355 -14.85 -91.07 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -14.85 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_1PG4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PG4-query.scw PDB file : Tito_Scwrl_1PG4.pdb: