Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDSQQSKPKHVMMMAAGTGGHVFPALAVAKQLQQQGCQVSWLATPIGMENRLLKDQNIPIYQIDIQGVRGN-G---V--------------IRKLAAP---FK-ILKATFSAMRYMKQLKVDAVAGFGGYVAGPGGLAARLLGIPVLIHEQNAVAGF---------TN----AQLSRVAKVVCEAFPNT----FPAS---EKVVTTGNPVRREITDILSPKWRYDEREQ-AGKPLNILIVGGSLGAK--ALNERLPPALKQLQVPLNIFHQCGQQQVEATQALYADAPANLTVQVLPFIEDMAKAYSEADLIICRAGALTVTEVATAGVAAVFVPLPIAVDDHQTANAKFLADVGAAKICQQSTMTPEVLNQLFTTLMNRQLL-TEMAVKARQHAQPNATQHVVGLIQKM 3OTH Chain:A ((14-404)) --GHIEGRHMRVLFASLGTHGHTYPLLPLATAARAAGHEVTFATGEGFA--GTLRKLGFEPVATGMPVFDGFLAALRIRFDTDSPEGLTPEQLSELPQIVFGRVIPQRVFDELQPVIERLRPDLVVQEIS--NYGAGLAALKAGIPTICHGVGRDTPDDLTRSIEEEVRGLAQRLGLDLPPGRIDGFGNPFIDIFPPSLQEPEFRARPRRHELRPVPFAEQGDLPAWLSSRDTARPLVYLTLGTSSGGTVEVLRAAIDGLAGL--DADVLVASGPSLDV-SG--LGEVPAN--VRLESWV-PQAALLPHVDLVVHHGGSGTTLGALGAGVPQLSFPWAG----DSFANAQAVAQAGAGDHLLPDNISPDSVSGAAKRLLAEESYRAGARAVAAEIAAMPGPDEVVRLLP--

General information: TITO was launched using: RESULT: Template: 3OTH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1941 -36957 -19.04 -107.12 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -19.04 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_3OTH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OTH-query.scw PDB file : Tito_Scwrl_3OTH.pdb: