Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKLIRADQYTKMLWKNGAGFTLEIARSQGEADFEWRISMADVTTSGPFSLFPNKQRIISVLDGQGMVLHVDDLPAKKLKQGDIFAFHGESQVQSELVDGAIRDLNLIYDPAKFHARFQWLNEAAEQAFISSADLIFIFNQGDETEVNVDEHSVQLAAHETLKIEKKSGVTSINFPKKQFKCCYIIELIQR 5IK2 Chain:P ((1-134)) -MATVQVDIVTP-----------ERKVFQGEADIVIARG-----VEGELGVMAGHIPLVTPLKTAPVRIKQGD------KETLIAVSGGFLEVRPD-------KVNILADTAELPEEI-----AVEAAKKAKARHETILKRLDKTDKDYLRHKRALERAEVRLQVANSK----------------------

General information: TITO was launched using: RESULT: Template: 5IK2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 585 -38873 -66.45 -290.09 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain P : 0.63 3D Compatibility (PKB) : -66.45 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.153

(partial model without unconserved sides chains): PDB file : Tito_5IK2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IK2-query.scw PDB file : Tito_Scwrl_5IK2.pdb: