Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLKNAYIIDAIRTPFGRYAGGLASVRADDLGAVPIKALMQRNPNVDWEQVDDVIYGCANQAGEDNRNVGRMSALLAGLPYQVPATTINRLCGSSLDAIAIAARAIKAGEANLVIAGGVESMSRAPYVMGKSDSAFGRSQKIEDTTMGWRFINPKLKELYGVDTMPQTAENVAEQFNVNRADQDQFALVSQQRTASAQAKGFFSKEIVAVEIPQRKGDAVVIDTDEHPRASTTLEGLSKLKPVVKADGTVTAGNASGINDGAAALLIASDDAVQAYNLKPRAKIIASTAVGVEPRIMGFAPAPAIKKLFKQANLTLDQMDVIELNEAFAAQALAVTRDLGLPDNSDKVNPNGGAIALGHPLGASGARLVTTALNQLEQTGGRYALCSMCIGVGQGIALIVERVEAL 4O9C Chain:H ((1-392)) --MTDVVIVSAARTAVGKFGGSLAKIPAPELGAVVIKAALER-AGVKPEQVSEVIMGQVLTAG-SGQNPARQAAIKAGLPAMVPAMTINKVSGSGLKAVMLAANAIMAGDAEIVVAGGQENMSAAPHVLPGSRDGF----RMGDAKLVDTMIVDGLWDVYNQYHMGITAENVAKEYGITREAQDEFAVGSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEFVRQGATLDSMSGLKPAFDKAGTVTAANASGLNDGAAAVVVMSAAKAKELGLTPLATIKSYANAGVDPKVMGMGPVPASKRALSRAEWTPQDLDLMEINEAFAAQALAVHQQMGW--DTSKVNVNGGAIAIGHPIGASGCRILVTLLHEMKRRDAKKGLASLCIGGGMGVALAVER----

General information: TITO was launched using: RESULT: Template: 4O9C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 2740 -225065 -82.14 -574.15 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain H : 0.84 3D Compatibility (PKB) : -82.14 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_4O9C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O9C-query.scw PDB file : Tito_Scwrl_4O9C.pdb: