Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQFAVIGLGSFGATVAQELTRLNHDVIGIDTVKKNVENLADVLTHAVIADATDEHVLDELNIQNCDAVVVAIGEDIEASILCVLNLKNLGIDKIWVKAKTKAHHTILSHLNINKIIHPEEDMGVRVAQALNYPMVSRYMSLDDEHYIVKIDIPEKLNGINLYGIMQQAPQVKTLLVKRQQQILFETEETFTLQTHDILILEGHLQQLKKLSNCFI 2HMU Chain:B ((8-141)) --QFAVIGLGRFGGSIVKELHRMGHEVLAVDINEEKVNAYASYATHAVIANATEENELLSLGIRNFEYVIVAIGANIQASTLTTLLLKELDIPNIWVKAQNYYHHKVLEKIGADRIIHPEKDMGVKIAQSLSDENV--------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2HMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 600 -118941 -198.23 -887.62 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -198.23 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.644

(partial model without unconserved sides chains): PDB file : Tito_2HMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HMU-query.scw PDB file : Tito_Scwrl_2HMU.pdb: