Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLIIGIDPGSRLTGYGIIEKDGS-KLRFVDAGTIRTET----------QEMPERLKRIFAGV-ERIVKFHGPTEAAVEQVFMAQNPDSA-----LKLGQARGAAIAALVNL---DLQVAEYTARQIKQSVVGYG-AADKEQVQMMVMRLLNLT------------------IKPQ-ADAADALAAAICHAHASGSMSKLTVLNALGGMARGRSRSSSRRR 1KCF Chain:A ((40-244)) -SRVLGIDLGIKNFSYCFASQNEDSKVIIHNWSVENLTEKNGLDIQWTEDFQPSSMADLSIQLFNTLHEKFNPHVILMERQRYRSG-IATIPEWTLRVNMLESMLYALHYAEKRNYPFLLSLSPKSTYSYWASVLN---KKSRVQMVKELIDGQKILFENEEALYKWNNGSRVEFKKDDMADSALIASGWMRWQAQ-------------------------

General information: TITO was launched using: RESULT: Template: 1KCF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 553 -1104 -2.00 -7.31 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -2.00 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_1KCF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KCF-query.scw PDB file : Tito_Scwrl_1KCF.pdb: