Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSLSLLHPYPFEKLNQLFKDTTPANLPLIPLSIGEPKHPAPEFVKQAIIDNFN--HLSTYPNSKGLPELRQSIADWLTKRFKLNSISAENHILPVSGTREGIFSFVQTLINREDAPYVVMPNPFYQIYE-GAALLAGAKPYFINCTEE---------NGYLGDFDAVPAEVWEKTALLFVCTPGNPTGTVLSKEQFKKLIALSDQYNFVIASDECYSELWFD-QAPTGLLEVCAELGRGDYKNCIVFHSLSKRSNLPGMRSGFIAGDAALLKPYLQYRTYHGAAMPVQHQLASIAAW-ND------E-SHVEENRKQYRAKFDLFQSEL-GHLLPLQKPDAGFYYWLKVDND--------------ETFAKMLMEKAHIKVLPGRYLSRDTEQGNPGANHVRMALVADLAQCEEVVKRLKAIL 3E2Y Chain:B ((4-410)) ---RIEGLDSNVWVEFT-KLA----ADPSVVNLGQGFPDISPPSYVKEELSKAAFIDNMNQYTRGFGHPALVKALSCLYGKIYQR-QIDPNEEILVAVGAYGSLFNSIQGLVDPGD--EVIIMVPFYDCYEPMV-RMAGAVPVFIPLRSKPTDGMKWTSSDWTFDPRELESKFSSKTKAIILNTPHNPLGKVYTRQELQVIADLCVKHDTLCISDEVYEWLVYTGHTHVKIATL-----PGMWERTITIGSAGKTFSVTGWKLGWSIGPAHLIKHLQTVQQNSFYTCATPLQAALAEAFWIDIKRMDDPECYFNSLPKELEVKRDRMVRLLNSVGLKPIVPDGGYFIIADVSSLGADLSDMNSDEPYDYKFVKWMTKHKKLTAIPVSAFCDSKSK-PHFEKLVRFCFIKKDSTLDAAEEIFRAWN

General information: TITO was launched using: RESULT: Template: 3E2Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1974 -32474 -16.45 -87.53 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -16.45 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_3E2Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E2Y-query.scw PDB file : Tito_Scwrl_3E2Y.pdb: