Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTFTLFFGVP-SGCSFGSIVALEWSGLPYQLCRIEMP-AQCNTEEYRQINPVGETPALLYDN-GKVLTESMAILNHIMAKSLNAAFHYEQGTEQFNLFNQKLAFLNTTLFNAFSPHWYSL-EHSSSEGNKQALREYGAVQVKKAFTALENSLTNQTWLMGDQLSFVDGYYAGIARWLKYHNVIDIDQFPNCKRLYEQLQNEAAVKFAHAIEQE 4KH7 Chain:B ((22-227)) --SMITLWGRNNSTNVKKVLWTLEELELPYDQILAGGKFGVNQDADYLAMNPNGLVPLLKDDETDLLLWESNAIVRYLAAQYGQNRLWVD-NPARRAEGEKWMDWANQTLSPAHRVILMGLVRTPP-EKRDQAAIEAGIEKCDSLFALLDDALAHQPWFSGDNFGTGDIAIAPFVYNLLNVGL-KWTPRPNLQRWYQQLTERPAFRKVVMI---

General information: TITO was launched using: RESULT: Template: 4KH7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 936 -34416 -36.77 -170.38 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -36.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_4KH7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KH7-query.scw PDB file : Tito_Scwrl_4KH7.pdb: