Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTRHFSQLPKAALAYPKVVQGLIFKKPKGPKILPQVEYVVDTLEIDQNHLKAYNEVCGFQNN----------GFV-PAIYLAVLSQSLQMH-MMTAEAFPFPILGLVHIRNQIKQTRPI-GVTEKLTLSCKLGELKPHDKGVQFDFITTAK--VGNEVVMEGLTTYLSRQKVEKRVGEK--A-----K-EEQAPAYVPKAEWNILENTGRRYAKVSGDFNLIHIHAITAKAFGFKQAIAHGMWSKAKALANLE-L-PNAYEADVWFKLPMFLPSKVEFLTANAD-KKTDFLIRNAKSQKPHVAGTVKAL 1PN2 Chain:C ((5-272)) -----------------------------------------PVWRFDDRDVILYNIALGATTKQLKYVYENDSDFQVIPTFGHLITFNS---NSFAKLLRNFNPMLLLHGEHYLKVHSWPPPTEGEIKTTFEPIATTPKGTNVVIVHGSKSVDNKSGELIYSNEATYFIRNCQADNKVYADRPAFATNQFLAPKRAPDYQVDVPVS-EDLAALYRLSGDRNPLHIDPNFAKGAKFPKPILHGMCTYGLSAKALIDKFGMFNEIKARFTGIVFPGETLRVLAWKESDDTIVFQTHVVDRGTIAINNAAIK-

General information: TITO was launched using: RESULT: Template: 1PN2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1173 3762 3.21 15.81 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain C : 0.62 3D Compatibility (PKB) : 3.21 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_1PN2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PN2-query.scw PDB file : Tito_Scwrl_1PN2.pdb: