Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKDIKVDPAQLEAALDARLKIRAGFDKPTAGMAAGMTQVNMISVPRDWAYDFLL-YAHRNPQSCPVLDVLEEGIYATKLAADSDIRTDFPRYRIWKDGEMVDEVTDAREIYNAHPDLVTFLIGCSFSFETALQEAGIEVRHIHDDTNVPMYLSNIKCEPAGRISGNMVVSMRPIPSHQISEAVKITARMPSVHGAPVHIGHPESLGIKDVNKPDFGDASRIEAGEIPVFWACGVTPQAAVINSKIPFAISHAPGYMFITDIPDRAWMG 5LZ3 Chain:B ((12-34)) --------------------------------------------IPLEYADDLLEAMAHHRPVPCSL----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5LZ3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 8 -1906 -238.19 -86.61 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain B : 0.52 3D Compatibility (PKB) : -238.19 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.233

(partial model without unconserved sides chains): PDB file : Tito_5LZ3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LZ3-query.scw PDB file : Tito_Scwrl_5LZ3.pdb: