Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDFLSEHLIYRDDDFMVIHKPSDILSVPGKGDLHDSVLTRLVAI------EPRTLLIHRLDRDTSGILVFGLSKRGQSAIARQFQDRQTSKIYEALVAGHLEGEGTVDIPVVYDPSRPPLHIVDASHNKPALTHWQAIEHFQIQGQPVTRVKLTPITGRSHQLRVHMQYLGHPIVGDTLYATPEQL-------------LVPRLCLHAKQLSFNHPVTGESLTFNCPVPF 1V9F Chain:A ((81-302)) ----IPLDIVYEDEDIIIINKPRDLVVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVESLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVH-PMGKPAVTHYRIMEHF----RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDRQALHATMLRLYHPISGIEMEWHAPIPQ

General information: TITO was launched using: RESULT: Template: 1V9F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1028 -19082 -18.56 -94.00 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -18.56 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_1V9F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V9F-query.scw PDB file : Tito_Scwrl_1V9F.pdb: