Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------MMRRLSTQDQNFKQVFAELLAFETVNDPNLVQTVDQIIADVRQHGDDHVLKLTQQFDRHPAHQFSDLELSQQQLKAAFDGLN------------------QEIREALQLAADRIRTFHEAQKQ-----DSWTYVDDLGNT------------LGQKVTPLDRVGIYVPGGLASYPSSVLMNAVPAHVAGV-----PEIIMVVPAPNGELNPLVLAAAYLA----GVSRVFTIGGAQAVAALAYGTQTVPRVDKITGPGNRFVAAAKRAVFGQLGIDMIAGPSEILVYAEG----VNN----AEWLAMDVLSQAE-HDTVAQAIFITPDGALLEAV---ESAIEKHLNELPKAEIARTSIENRGALVLVKDRAEAVDLINQVAPEHLELCLDDAEAMSQDIRHAGAIFMGRYTPEAIGDYCAGPNHVLPTSGTARFSSPLGVYDFQKRSSLIMCSKDGVKTLAKTADVLAVQENLDAHARSARYRYQ 4ZLA Chain:A ((7-502)) MLKIKLEKTTFENAKAECSLVFIINKDFSHAWVKNKELLETFKYEGEGVFLDQENKILYAGVKEDDVHLLRESACLAVRTLKKLAFKSVKVGVYTCGAH-----NALLENLKALFLGLKLGLYEYDTFKSNKKESVLKEAIVALELHKSLEKSAKEALKYAEIMTESLNIVKDLVNTPPMIGTPVYMAEVAQKVAKENHLEIHVHD--EKFLEEKKMNAFLAVNKASLSVNPPRLIHLVYKPKKAKKKIALVGKGLTYDCGGLSLKPADYMVTMKADKGGGSAVIGLLNALAKLGVEAEVHGIIGATENMIGPAAYKPDDILISKEGKSIEVRNTDAEGRLVLADCLSYAQDLNPDVIVDFATLTGACVVGLGEFTSAIMGH-NEELKNLFETSGLESGELLAKLPFNRHLKKLIESKIA--------DVCNISSS-RYGGAITAGLFLNEFIRDEFKDKWLHIDIAGPAYVEKEWDVNSF-------GASGAGVRACTAFVEELLKKA--------------

General information: TITO was launched using: RESULT: Template: 4ZLA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2122 29801 14.04 76.02 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 14.04 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.187

(partial model without unconserved sides chains): PDB file : Tito_4ZLA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZLA-query.scw PDB file : Tito_Scwrl_4ZLA.pdb: